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 Design / Assignment: Name a Gene Drive!
Read the assigned preprint titled: “Daisy Quorum Drives for the Genetic Restoration of Wild Populations” 


https://www.biorxiv.org/content/early/2017/03/21/115618.full.pdf+html


In your own words, please answer the questions below:

 

1. What is the purpose of “Daisy Quorum” gene drive?

 

 The purpose of "Daisy Quorum" is allow efficient, community-supported and genetically reversible ecological engineering. In other words, Daisy Quorum purposes a responsible use by allowing local communities to decide how to solve their own ecological problem.

 

 

2. What is your immediate emotional response after reading the preprint?

Let´s do it!

 

 

 

 

 

 

 

 

 

 

 

 

 

3. Describe a scenario, real or fictional (real-world is preferred), where you would deploy a technology such as “Daisy Quorum”.

 

Considering that Paraguay is the 6th biggest soybean producer, Sabrina and I thought about applying the Daisy quorum drive system to solve the insect infestation of soybean crops in our country. This concerning a socio-economic interest that could help improve the national agriculture yield.

 

4. What would you be concerned about if a neighboring country, or city, decided to deploy “Daisy Quorum” technology?

 

If a neighboring country decided to implement this technology, as scientists we know it went through a long stage to be approved and put into operation, so I am sure that the development of this technology will be as safe and ethical as possible.

 

 

5. Is the name “Daisy Quorum” intuitive to you? If you were to name the technology, what would you name it? Note: we may actually adopt your name in the final publication!

 

The name was not very intuitive to me, but once I read the paper I could understand why.

 

 

 

 

 

 



Experimental Assignment:

 

 

 

Imagine all the people living life in peace...

Imagine a world without mosquitoes that transmit diseases, without pests in crops...

Imagine a world that precisely solves ecological problems using biology itself.

For this experimental assignment, we transformed the bacteria with the EC-Rope plasmid and plated on selective LB-Agar with 25 ug/mL of Carbenicillin. Then we made new competent cells that contain the EC-Rope plasmid.

 

Also, we mixed the following plasmid:

 

a. CRISPR-A: Ds-cas9 + PM-bla

b. CRISPR-B: Ds-cas9 + PM-sp22a-!con

After that, we transformed two aliquots of competent cells with the two plasmid mixtures from the last step. Finally, we plated cells on double selective LB-Agar plates containing Spectinomycin at 50ug/mL, and Chloramphenicol at 35 ug/mL and pick out colonies and streak them out on LB-Agar with 25ug/mL.

 

The colonies carrying the CRISPR-B mix grow and the CRISPR-A mix fail.

GENE DRIVES

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