GUT MICROBIOME
Homework
Culture bacteria from your skin.
We prepared LB plates agar and swab our hands surfaces with a moistened sterile swab and gently brush the swap on the agar
We incubate at room temperature for 2 days
This is a small test of the bacteria that abound in nature and in our body. Having these bacteria on our hands is normal and we are in contact with them daily.
3D print a 14 mL culture tube in at least one material.
Design a milli- or micro-fluidic 'artificial gut' or other 'organ-on-a-chip' device to be utilized, at a minimum, for cell culture.
For this assignment, I tried to design a microfluidic photobioreactor that consists of a microalgae culture layer to produce biomass.
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The use of microfluidic bioreactor platforms for cell culturing holds considerable promise for a range of fields which include drug discovery, tissue engineering, bioprocessing optimization and cell-based screening studies
The developed microfluidic photobioreactor provides a promising prototype for a cost-effective for obtaining biomass. Conventional laboratory methods are time-consuming and labor-intensive, requiring expensive and bulky equipment.
In view of this, microfluidic bioreactors have attractive features which make them ideal to study the behavior of cells and their internal organization in their native microenvironment
In the development of growth medium formulations and strain improvements cells can be cultured in parallel microbioreactor platforms to find the best combination of nutrients, including sugars, amino acids, vitamins, minerals and hormone
In the design and development of effective microbioreactor platforms, a number of aspects need to be considered. These include; material compatibility, mechanical (shear stress), mass and heat balance, physicochemical factors (oxygen tension, pH, CO2, temperature), fluidic, sensing and control elements.
ODs of each tube was measured like this:
E.coli turbo cells were grown for 24 hours in a shaking incubator at 37C in one standard culture tube and two other 3D printed tubes of the same dimensions as a standard one. 5 ml of culture is grown in each using LB 1x. The OD is measured by transferring 100ul of each culture and 900ul of LB 1x to a cuvette. In total 3 cuvettes for each culture and one cuvette full of 1000ul LB 1x. The measured ODs for these diluted cultures are 0.57, 0.45, 0.37 and 0 for LB 1x cuvette. These ODs are for diluted cultures. The original culture has 5.7, 4.5, 3.7. We had to dilute the culture because OD measurement is good between 0 to 1.5 after that usually it's erroneous. That's why we dilute it and measure it and then scale it back.